I'm currently writing a Lichess study on the Scotch Gambit/Two Knights Defence position that arises after 1.e4 e5 2.Nf3 Nc6 3.Bc4 Nf6 4.d4 exd4 (or 3.d4 exd4 4.Bc4 Nf6).
I don't think I have previously covered that line in much detail, but it has emerged as a serious alternative to the main lines (8...Qa5, 8...Qh5). It aims to improve over the 8...Qd8 line (Black probably isn't fully equalising after 8...Qd8 9.Rxe4+). Graham Burgess gave it a good look in his updated version of Chris Baker's A Startling Chess Opening Repertoire. After 8...Qd7 9.Nxe4 White should be no worse, as White can put the knights onto active posts so that they aren't inferior to Black's knight and bishop, but it is trickier to generate winning chances.
Via a search of the ChessBase database, I have also found a new try for White in the main line of the Canal Variation (5.0-0 Nxe4 6.Re1 d5 7.Nc3 dxc3 8.Bxd5 Be6 9.Bxe4 Qxd1 10.Rxd1 cxb2 11.Bxb2 f6 12.h4) which complicates matters and so could be a good practical try for rapid games and especially online blitz, though I still think Black's better with best play in that variation and that 7.Nc3 is therefore not as reliable as 7.Bxd5.
My fresh investigations into the 5.e5 lines are only just starting, so I'll have to see if anything new turns up in those.